ANXA5 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPP4; ANX5; ENX2; RPRGL3; HEL-S-7
ProductOrderA
Clone#3B9B7
Entrez GenelD308
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ANXA5 (AA: 160-320) expressed in E. Coli.
MW36kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
160-320
Catalog#: 30796
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)
Figure 2:Western blot analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)
Western Blot
Figure 3:Western blot analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate.
Figure 4:Western blot analysis using ANXA5 mouse mAb against HepG2 (1), PNAC-1 (2), NIH/3T3 (3), Hela (4), MCF-7 (5), and A431 (6) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using ANXA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using ANXA5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).
Flow cytometric
Figure 7:Flow cytometric analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of MCF-7 cells using ANXA5 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThe protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
References (references)
References (references)1.Klin Lab Diagn. 2013 Apr;(4):9-10.
2.Mech Ageing Dev. 2012 Jul;133(7):508-22.