ANXA5 Primary Antibody

Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
Product Overview
Entrez GenelD
308
Aliases
PP4; ANX5; ENX2; RPRGL3; HEL-S-7
Clone#
3B9F3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ANXA5 (AA: 160-320) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
Klin Lab Diagn. 2013 Apr;(4):9-10.
Mech Ageing Dev. 2012 Jul;133(7):508-22.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2: Western blot analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)
Figure 2: Western blot analysis using ANXA5 mAb against human ANXA5 (AA: 160-320) recombinant protein. (Expected MW is 44.1 kDa)
Western Blot
Figure 3:Western blot analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using ANXA5 mAb against HEK293 (1) and ANXA5 (AA: 160-320)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ANXA5 mouse mAb against PANC-1 (1), Hela (2), A431 (3), A549 (4) and MCF-7 (5) cell lysate.
Figure 4:Western blot analysis using ANXA5 mouse mAb against PANC-1 (1), Hela (2), A431 (3), A549 (4) and MCF-7 (5) cell lysate.
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using ANXA5 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.