ANPEP Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAPN; CD13; LAP1; P150; PEPN; GP150
ProductOrderA
Clone#1C7D7
Entrez GenelD290
FormulationPurified antibody in PBS with 0.05% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ANPEP (AA: Extra(781-967)) expressed in E. Coli.
MW110kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
Extra(781-967)
Catalog#: 30732
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Images
Western Blot
Figure 1: Western blot analysis using ANPEP mAb against human ANPEP (AA: Extra(781-967)) recombinant protein. (Expected MW is 47.4 kDa)
Figure 1: Western blot analysis using ANPEP mAb against human ANPEP (AA: Extra(781-967)) recombinant protein. (Expected MW is 47.4 kDa)
Western Blot
Figure 2: Western blot analysis using ANPEP mAb against HEK293 (1) and ANPEP (AA: Extra(781-967))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using ANPEP mAb against HEK293 (1) and ANPEP (AA: Extra(781-967))-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using ANPEP mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using ANPEP mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma.

References (references)
References (references)1. Br J Cancer. 2013 Feb 5;108(2):420-8.
2. Ann Surg Oncol. 2012 Jul;19 Suppl 3:S539-48.