ANAPC1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation.

Product Overview

Entrez GenelD

64682

Aliases

APC1; MCPR; TSG24

Clone#

7G9B3

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human ANAPC1 (AA: 12-155) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Drug Alcohol Depend. 2012 Aug 1;124(3):325-32.
2.Braz J Med Biol Res. 2008 Jun;41(6):539-43.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using ANAPC1 mAb against human ANAPC1 (AA: 12-155) recombinant protein. (Expected MW is 41.9 kDa)

Western Blot

Figure 3:Western blot analysis using ANAPC1 mAb against HEK293 (1) and ANAPC1 (AA: 12-155)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using ANAPC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric