AMY1A Primary Antibody

Item Information
Catalog #
Size
Price
Description
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein.
Product Overview
Entrez GenelD
276
Aliases
AMY1
Clone#
1G4D8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human AMY1A (AA: 172-284) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Physiol Behav. 2015 Jan;138:173-8.
2.Am J Surg Pathol. 2013 Dec;37(12):1824-30.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AMY1A mAb against human AMY1A (AA: 172-284) recombinant protein. (Expected MW is 38.7 kDa)
Figure 2:Western blot analysis using AMY1A mAb against human AMY1A (AA: 172-284) recombinant protein. (Expected MW is 38.7 kDa)
Western Blot
Figure 3:Western blot analysis using AMY1A mAb against HEK293 (1) and AMY1A (AA: 172-284)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using AMY1A mAb against HEK293 (1) and AMY1A (AA: 172-284)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of MCF-7 cells using AMY1A mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of MCF-7 cells using AMY1A mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.