AMBRA1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity).
Product Overview
Entrez GenelD
55626
Aliases
DCAF3; WDR94
Clone#
6B11D1
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human AMBRA1 (AA: 1177-1301) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Dev Cell. 2018 Dec 3;47(5):592-607. 2.Oncogene. 2013 Jul 11;32(28):3311-8.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using KDM1A mAb against human KDM1A (AA: 709-876) recombinant protein. (Expected MW is 43.8 kDa)
Western Blot
Figure 3:Western blot analysis using KDM1A mAb against HEK293 (1) and KDM1A (AA: 709-876)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using KDM1A mouse mAb against COS7 (1), NIH/3T3 (2), and A549 (3) cell lysate.
Flow Cytometric
Figure 5:Flow cytometric analysis of Hela cells using KDM1A mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using KDM1A mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KDM1A mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.