Mouse Monoclonal Antibody to ALPG

Item Information

Catalog #

Size

Price

Description

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.

Product Overview

Entrez GenelD

251

Aliases

GCAP; ALPPL; ALPPL2

Clone#

3A2B7

Host / Isotype

Mouse / IgG2b

Immunogen

Purified recombinant fragment of human ALPG (AA: 170-285) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/50 - 1/200

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cancer Res. 2020 Oct 15;80(20):4552-4564.2.Cell Rep. 2020 Mar 17;30(11):3917-3931.e5.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using ALPG mAb against human ALPG (AA: 170-285) recombinant protein. (Expected MW is 38.8 kDa)

Western Blot

Figure 3:Western blot analysis using ALPG mAb against HEK293-6e (1) and ALPG (AA: 170-285)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using ALPG mouse mAb against SK-OV-3 (1), Hela (2),HepG2 (3), and A431 (4) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using ALPG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis