Mouse Monoclonal Antibody to ALPG
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Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
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Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ALPG mAb against human ALPG (AA: 170-285) recombinant protein. (Expected MW is 38.8 kDa)
Western Blot
Figure 3:Western blot analysis using ALPG mAb against HEK293-6e (1) and ALPG (AA: 170-285)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ALPG mouse mAb against SK-OV-3 (1), Hela (2),HepG2 (3), and A431 (4) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using ALPG mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using ALPG mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALPG mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ALPG mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.