Mouse Monoclonal Antibody to ALK/p80

Item Information
Catalog #
Size
Price
Description

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).

Product Overview
Entrez GenelD
238
Aliases
CD246; NBLST3
Clone#
8C9A2
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human ALK/p80 (AA: 1359-1460) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.BMC Cancer. 2020 May 27;20(1):479. 2.Pathol Res Pract. 2019 Dec;215(12):152669.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ALK/p80 mAb against human ALK/p80 (AA: 1359-1460) recombinant protein. (Expected MW is 37.3 kDa)
Figure 2:Western blot analysis using ALK/p80 mAb against human ALK/p80 (AA: 1359-1460) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
Figure 3:Western blot analysis using ALK/p80 mAb against HEK293-6e (1) and ALK/p80 (AA: 1359-1460)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using ALK/p80 mAb against HEK293-6e (1) and ALK/p80 (AA: 1359-1460)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ALK/p80 mouse mAb against SK-N-SH (1) cell lysate.
Figure 4:Western blot analysis using ALK/p80 mouse mAb against SK-N-SH (1) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of *** cells using *** mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of *** cells using *** mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALK/p80 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALK/p80 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded stomach tissues using ALK/p80 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded stomach tissues using ALK/p80 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 32089

Inquire
Contact us to order bulk/custom
1-866-339-0871
510-740-3625
info@promab.com