ALDH6A1 Primary Antibody
This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded protein is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants.
Purified antibody in PBS with 0.05% sodium azide
4°C; -20°C for long term storage
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using ALDH6A1 mAb against human ALDH6A1 (AA: 1-195) recombinant protein. (Expected MW is 47.2 kDa)
Figure 3:Western blot analysis using ALDH6A1 mAb against HEK293 (1) and ALDH6A1 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Western blot analysis using ALDH6A1 mouse mAb against Jurkat (1), HEK293 (2), Hela (3), MCF-7 (4), and LNcap (5) cell lysate.
Figure 5:Immunofluorescence analysis of Hela cells using ALDH6A1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Figure 6:Immunofluorescence analysis of Hela cells using ALDH6A1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 7:Flow cytometric analysis of Hela cells using ALDH6A1 mouse mAb (green) and negative control (red).
Figure 8:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ALDH6A1 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using ALDH6A1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.