AKT1S1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]
Product Overview
Entrez GenelD
84335
Aliases
Lobe; PRAS40
Clone#
4A11G7
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human AKT1S1 (AA: 92-276) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Cell Biol. 2017 Sep 12;37(19). 2.Arch Physiol Biochem. 2014 May;120(2):64-72.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AKT1S1 mAb against human AKT1S1 (AA: 92-276) recombinant protein. (Expected MW is 45.9 kDa)
Western Blot
Figure 3:Western blot analysis using AKT1S1 mAb against HEK293 (1) and AKT1S1 (AA: 92-276)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using AKT1S1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.