AKT1 Primary Antibody

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Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

Product Overview
Entrez GenelD
207
Aliases
AKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA; AKT1
Clone#
3A3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey
Immunogen
Purified recombinant fragment of human AKT1 expressed in E. Coli.

Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. J Cell Physiol. 2010 Oct;225(1):168-73.
2. Mol Cell Biol. 1995 Apr;15(4):2304-10.
3. EMBO J. 1997 Sep 1;16(17):5445-54.
Product Image
Western Blot
Figure 1: Western blot analysis using AKT1 mouse mAb against NIH/3T3 (1)? Hela (2)?COS7 (3) and Jurkat (4) cell lysate.
Figure 1: Western blot analysis using AKT1 mouse mAb against NIH/3T3 (1)? Hela (2)?COS7 (3) and Jurkat (4) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.