AKT1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAKT; PKB; RAC; PRKBA; MGC99656; PKB-ALPHA; RAC-ALPHA; AKT1
ProductOrderA
Clone#3A3
Entrez GenelD207
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human AKT1 expressed in E. Coli.

MW56kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Monkey
Application
ELISA1/10000
WB (Western Blot)1/500 - 1/2000
Sequence
N
Catalog#: 20434
Inquire
Contact us to order
Images
Western Blot
Figure 1: Western blot analysis using AKT1 mouse mAb against NIH/3T3 (1)? Hela (2)?COS7 (3) and Jurkat (4) cell lysate.
Figure 1: Western blot analysis using AKT1 mouse mAb against NIH/3T3 (1)? Hela (2)?COS7 (3) and Jurkat (4) cell lysate.
Product Overview
Description

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.

References (references)
References (references)1. J Cell Physiol. 2010 Oct;225(1):168-73.
2. Mol Cell Biol. 1995 Apr;15(4):2304-10.
3. EMBO J. 1997 Sep 1;16(17):5445-54.