AIM2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

AIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.

Product Overview

Entrez GenelD

9447

Aliases

PYHIN4

Clone#

7G12D1

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human AIM2 (AA: 1-195) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1.Int J Cancer. 2014 Nov 15;135(10):2387-96.
2.Mol Cancer Res. 2013 Oct;11(10):1193-202.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using AIM2 mAb against human AIM2 (AA: 1-195) recombinant protein. (Expected MW is 48.2 kDa)

Western Blot

Figure 3:Western blot analysis using AIM2 mAb against HEK293 (1) and AIM2 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using AIM2 mouse mAb against HepG2 (1) and A549 (2) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

For Research Use Only. Not for use in diagnostic procedures.