AIM2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPYHIN4
ProductOrderA
Clone#3C4G11
Entrez GenelD9447
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human AIM2 (AA: 1-195) expressed in E. Coli.
MW39kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-195
Catalog#: 30795
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Images
Western Blot
Figure 1:Western blot analysis using AIM2 mAb against human AIM2 (AA: 1-195) recombinant protein. (Expected MW is 48.2 kDa)
Figure 1:Western blot analysis using AIM2 mAb against human AIM2 (AA: 1-195) recombinant protein. (Expected MW is 48.2 kDa)
Elisa
Figure 2:Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 2:Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 3:Western blot analysis using AIM2 mAb against HEK293 (1) and AIM2 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using AIM2 mAb against HEK293 (1) and AIM2 (AA: 1-195)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of A549 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of A549 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of MCF-7 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of MCF-7 cells using AIM2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of Hela cells using AIM2 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Hela cells using AIM2 mouse mAb (green) and negative control (red).
Product Overview
DescriptionAIM2 is a member of the IFI20X /IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2.
References (references)
References (references)1.Mol Cancer Res. 2013 Oct;11(10):1193-202.
2.Int J Cancer. 2010 Apr 15;126(8):1838-49.