AHNAK

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesPM227; AHNAKRS
Clone#8B3A4
Entrez GenelD79026
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human AHNAK (AA: 1-200) expressed in E. Coli.
MW63KDa
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-200
Catalog#: 32277
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510-740-3625
info@promab.com
Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AHNAK mAb against human AHNAK (AA: 1-200) recombinant protein. (Expected MW is 25.2 kDa)
Figure 2:Western blot analysis using AHNAK mAb against human AHNAK (AA: 1-200) recombinant protein. (Expected MW is 25.2 kDa)
Western Blot
Figure 3:Western blot analysis using AHNAK mAb against HEK293-6e (1) and AHNAK (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using AHNAK mAb against HEK293-6e (1) and AHNAK (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using AHNAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using AHNAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hela cells using AHNAK mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Hela cells using AHNAK mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HepG2 cells using AHNAK mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HepG2 cells using AHNAK mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded Cervical cancer tissues using AHNAK mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded Cervical cancer tissues using AHNAK mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using AHNAK mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using AHNAK mouse mAb with DAB staining.
Product Overview
Description

The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene.

References (references)
References (references)1.Cancer Med. 2018 Oct;7(10):5178-5186.
2.J Proteome Res. 2021 May 7;20(5):2308-2318.
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