AFP (alpha-fetoprotein) Primary Antibody
Item Information
Catalog #
Size
Price
Description
AFP is a major plasma protein in the fetus, binding to copper, nickel, fatty acids and bilirubin. In adults, alpha-fetoprotein levels are low but can be elevated by hepatocellular carcinoma.Â
Product Overview
Entrez GenelD
174
Aliases
AFPD; FETA; HPAFP
Clone#
2G4H3
Host / Isotype
Mouse / Mouse IgG2b
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human AFP (AA: 19-210) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.BMC Cancer. 2021 Jun 14;21(1):699.
2.Biomed Res Int. 2020 Sep 17;2020:5087643.
2.Biomed Res Int. 2020 Sep 17;2020:5087643.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AFP mAb against human AFP (AA: 19-210) recombinant protein. (Expected MW is 52.3 kDa)
Immunofluorescence analysis
Figure 3:Flow cytometric analysis of HepG2 cells using AFP mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded brain tissues using AFP mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using AFP mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.