AEBP2 Primary Antibody

Item Information
Catalog #
Size
Price
Description
AEBP2 (AE Binding Protein 2) is a Protein Coding gene. Among its related pathways are Chromatin organization and Activated PKN1 stimulates transcription of AR (androgen receptor) regulated genes KLK2 and KLK3. GO annotations related to this gene include RNA polymerase II core promoter proximal region sequence-specific DNA binding and transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding.
Product Overview
Entrez GenelD
121536
Aliases
N
Clone#
2D7B6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human AEBP2 (AA: 358-495) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/100 - 1/500
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2015 Apr 27;10(4):e0126966.
2.Elife. 2012 Oct 30;1:e00005.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AEBP2 mAb against human AEBP2 (AA: 358-495) recombinant protein. (Expected MW is 41.8 kDa)
Figure 2:Western blot analysis using AEBP2 mAb against human AEBP2 (AA: 358-495) recombinant protein. (Expected MW is 41.8 kDa)
Western Blot
Figure 3:Western blot analysis using AEBP2 mAb against HEK293 (1) and AEBP2 (AA: 358-495)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using AEBP2 mAb against HEK293 (1) and AEBP2 (AA: 358-495)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using AEBP2 mouse mAb against COS7 (1), HepG2 (2), and SK-MES-1 (3) cell lysate.
Figure 4:Western blot analysis using AEBP2 mouse mAb against COS7 (1), HepG2 (2), and SK-MES-1 (3) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using AEBP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using AEBP2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of MCF-7 cells using AEBP2 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of MCF-7 cells using AEBP2 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.