ADD1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
Product Overview
Entrez GenelD
118
Aliases
ADDA
Clone#
5D4H1
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ADD1 (AA: 1-193) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
EMBO J. 2012 Mar 21;31(6):1453-66.
Traffic. 2011 Oct;12(10):1327-40.
Traffic. 2011 Oct;12(10):1327-40.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using ADD1 mAb;against human ADD1 (AA: 1-193);recombinant protein. (Expected MW is 47.8 kDa)
Western Blot
Figure 3:Western blot analysis using ADD1 mAb ;against HEK293 (1) and ADD1 (AA: 1-193)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using 1-193 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using 1-193 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.