ADAM10 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.
Product Overview
Entrez GenelD
102
Aliases
RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
Clone#
6C3C7
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human ADAM10 (AA: EXTRA(20-119)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,Ann Clin Lab Sci. 2019 May;49(3):309-316.2,J Biol Chem. 2019 Apr 26;294(17):7085-7097.3,EMBO Mol Med. 2019 Apr;11(4). pii: e9695.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using ADAM10 mAb against human ADAM10 (AA: EXTRA(20-119)) recombinant protein. (Expected MW is37.7 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using ADAM10 mAb against HEK293-6e (1) and ADAM10 (AA: EXTRA(20-119))-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using ADAM10 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of THP-1 cells using ADAM10 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ADAM10 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 7: Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ADAM10 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.