ACTR3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Three transcript variants encoding two different isoforms have been found for this gene.
Product Overview
Entrez GenelD
10096
Aliases
ARP3
Clone#
3G2C9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human ACTR3 (AA: 287-418) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Immunol. 2014 Jul 1;193(1):150-60.
2.J Cell Biol. 2013 Dec 23;203(6):907-16.
2.J Cell Biol. 2013 Dec 23;203(6):907-16.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ACTR3 mAb against human ACTR3 (AA: 287-418) recombinant protein. (Expected MW is 41.1 kDa)
Western Blot
Figure 3:Western blot analysis using ACTR3 mAb against HEK293 (1) and ACTR3 (AA: 287-418)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using ACTR3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ACTR3 mouse mAb with DAB staining.
Western Blot
Figure 6:Western blot analysis using ACTR3 mouse mAb against NIH/3T3 (1) and A549 (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.