ACTH Primary Antibody
This gene encodes a preproprotein that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the preproprotein and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. The antimicrobial melanotropin alpha peptide exhibits antibacterial and antifungal activity. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
![Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-elisa_31934ea_483.jpg?v=1666704959)
![Figure 2: Western blot analysis using ACTH mAb against human ACTH recombinant protein. (Expected MW is 21.9 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31934rwb_484.jpg?v=1666704959)
![Figure 3: Western blot analysis using ACTH mAb against HEK293-6e (1) and ACTH-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31934twb_485.jpg?v=1666704959)
![Figure 4: Immunofluorescence analysis of Hela cells using ACTH mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-icc_31934icc_488.jpg?v=1666704959)
![Figure 5: Flow cytometric analysis of Hela cells using ACTH mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31934fcm-1_486.jpg?v=1666704959)
![Figure 6: Flow cytometric analysis of MCF-7 cells using ACTH mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31934fcm-2_487.jpg?v=1666704959)