ACTA2 Primary Antibody
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Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT

Figure 2: Western blot analysis using ACTA2 mAb against human ACTA2 (AA: 2-124) recombinant protein. (Expected MW is 40 kDa)
WESTERN BLOT

Figure 3: Western blot analysis using ACTA2 mouse mAb against EC (1), HUVE-12 (2), A549 (3), NIH/3T3 (4), HL-60 (5), Hela (6), and K652 (7) cell lysate.
FLOW CYTOMETRY

Figure 4: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY

Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ACTA2 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY

Figure 6: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ACTA2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.