ACTA2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.
Product Overview
Entrez GenelD
59
Aliases
ACTSA
Clone#
4G5D2
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human ACTA2 (AA: 2-124) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.BMC Med Genet. 2017 Dec 4;18(1):143. 2.Braz J Cardiovasc Surg. 2015 Nov-Dec;30(6):644-9.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using ACTA2 mAb against human ACTA2 (AA: 2-124) recombinant protein. (Expected MW is 40 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using ACTA2 mouse mAb against EC (1), HUVE-12 (2), A549 (3), NIH/3T3 (4), HL-60 (5), Hela (6), and K652 (7) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ACTA2 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using ACTA2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.