ACTA2 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesAAT6; ACTSA; α-Smooth Muscle Actin
ProductOrderA
Clone#4A4
Entrez GenelD59
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenSynthesized peptide of human ACTA2.
MW42kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse, Rat, Monkey
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
C-EYDEAGPS IVHRKCF
Catalog#: 30235
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Images
Western Blot
Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using ACTA2 mouse mAb with DAB staining.
Figure 2: Immunohistochemical analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using ACTA2 mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using ACTA2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Immunofluorescence analysis of HepG2 cells using ACTA2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Product Overview
Description

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

References (references)
References (references)1. J Hum Genet. 2009 Nov;54(11):687-8.
2. Hum Mutat. 2009 Oct;30(10):1406-11.
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