ACTA2 Primary Antibody

Item Information

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Price

Description

The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product Overview

Entrez GenelD

Aliases

AAT6; ACTSA; α-Smooth Muscle Actin

Clone#

4A4

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide of human ACTA2.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. J Hum Genet. 2009 Nov;54(11):687-8.
2. Hum Mutat. 2009 Oct;30(10):1406-11.

Product Image

Western Blot

Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) using ACTA2 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of HepG2 cells using ACTA2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Flow cytometric