ACTA2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Actin,alpha 2, smooth muscle, aorta, major constituent of thin filaments.
Product Overview
Entrez GenelD
59
Aliases
AAT6; ACTSA; ACTA2
Clone#
1H8
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey, Rat, Mouse
Immunogen
Purified recombinant fragment of human ACTA2 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Nat Genet. 2007 Dec;39(12):1488-93.
2. Virchows Arch. 2007 Dec;451(6):999-1007.
2. Virchows Arch. 2007 Dec;451(6):999-1007.
Product Image
Western Blot
Figure 1: Western blot analysis using ACTA2 mouse mAb against Hela (1), Jurkta (2), HepG2 (3), MCF-7 (4), A431 (5), A549 (6), PC-12 (7), NIH/3T3 (8) and Cos7 (9) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using ACTA2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using ACTA2 mouse mAb (green) and negative control (purple).
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.