ACADM Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
34
Aliases
MCAD; ACAD1; MCADH
Clone#
6F2G12
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ACADM (AA: 26-185) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biochemistry. 2016 Dec 27;55(51):7086-7098.
2.Pediatr Int. 2019 Jun;61(6):551-557.
2.Pediatr Int. 2019 Jun;61(6):551-557.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ACADM mAb against human ACADM (AA: 26-185) recombinant protein. (Expected MW is 20.5kDa)
Western Blot
Figure 3:Western blot analysis using ACADM mAb against HEK293-6e (1) and ACADM (AA: 26-185)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ACADM mouse mAb against Hela (1), HepG2 (2),Jurkat (3),Raji (4) and K562 (5) cell lysate.
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using ACADM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HL-60 cells using ACADM mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ACADM mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.