ACADM

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesMCAD; ACAD1; MCADH
Clone#6F2G12
Entrez GenelD34
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human ACADM (AA: 26-185) expressed in E. Coli.
MW46.6KDa
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
26-185
Catalog#: 32271
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ACADM mAb against human ACADM (AA: 26-185) recombinant protein. (Expected MW is 20.5kDa)
Figure 2:Western blot analysis using ACADM mAb against human ACADM (AA: 26-185) recombinant protein. (Expected MW is 20.5kDa)
Western Blot
Figure 3:Western blot analysis using ACADM mAb against HEK293-6e (1) and ACADM (AA: 26-185)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using ACADM mAb against HEK293-6e (1) and ACADM (AA: 26-185)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ACADM mouse mAb against Hela (1), HepG2 (2),Jurkat (3),Raji (4) and K562 (5) cell lysate.
Figure 4:Western blot analysis using ACADM mouse mAb against Hela (1), HepG2 (2),Jurkat (3),Raji (4) and K562 (5) cell lysate.
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using ACADM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using ACADM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HL-60 cells using ACADM mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HL-60 cells using ACADM mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ACADM mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ACADM mouse mAb with DAB staining.
Product Overview
Description

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1.Biochemistry. 2016 Dec 27;55(51):7086-7098.
2.Pediatr Int. 2019 Jun;61(6):551-557.