ABCB5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules
Product Overview
Entrez GenelD
340273
Aliases
ABCB5beta; EST422562; ABCB5alpha
Clone#
5H3C6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ABCB5 (AA: 481-674) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Gastroenterology. 2011 Jan;140(1):344-55.
2.Cancer Res. 2011 Aug 1;71(15):5307-16.
2.Cancer Res. 2011 Aug 1;71(15):5307-16.
Product Image
Western Blot
Figure 1: Western blot analysis using ABCB5 mAb against human ABCB5 recombinant protein. (Expected MW is 47 kDa)
Western Blot
Figure 2: Western blot analysis using ABCB5 mouse mAb against A431 (1) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using ABCB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of HepG2 cells using ABCB5 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ABCB5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ABCB5 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.