ABCB5 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesABCB5beta; EST422562; ABCB5alpha
ProductOrderA
Clone#5H3C6
Entrez GenelD340273
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human ABCB5 (AA: 481-674) expressed in E. Coli.
MW89.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
481-674
Catalog#: 30432
Inquire
Contact us to order
Images
Western Blot
Figure 1: Western blot analysis using ABCB5 mAb against human ABCB5 recombinant protein. (Expected MW is 47 kDa)
Figure 1: Western blot analysis using ABCB5 mAb against human ABCB5 recombinant protein. (Expected MW is 47 kDa)
Western Blot
Figure 2: Western blot analysis using ABCB5 mouse mAb against A431 (1) cell lysate.
Figure 2: Western blot analysis using ABCB5 mouse mAb against A431 (1) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using ABCB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HepG2 cells using ABCB5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of HepG2 cells using ABCB5 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of HepG2 cells using ABCB5 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ABCB5 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ABCB5 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ABCB5 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ABCB5 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
DescriptionABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules

References (references)
References (references)1.Gastroenterology. 2011 Jan;140(1):344-55.
2.Cancer Res. 2011 Aug 1;71(15):5307-16.