Mouse Monoclonal Antibody to ABCA1
Item Information
Catalog #
Size
Price
Description
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in both alleles of this gene cause Tangier disease and familial high-density lipoprotein (HDL) deficiency.
Product Overview
Entrez GenelD
19
Aliases
TGD; ABC1; CERP; ABC-1; HDLDT1; HPALP1; HDLCQTL13
Clone#
6B7A11
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human ABCA1 (AA: 2081-2261) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
References
1,Life Sci. 2020 Oct 15;259:118390. 2,Medicine (Baltimore). 2020 Jan;99(4):e18662.
Product Image
Western Blot
Figure 1:Western blot analysis using ABCA1 mAb against human ABCA1 (AA: 2081-2261) recombinant protein. (Expected MW is 46.4 kDa)
Western Blot
Figure 2:Western blot analysis using ABCA1 mAb against HEK293-6e (1) and ABCA1 (AA: 2081-2261))-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 3:Flow cytometric analysis of bel-7402 cells using ABCA1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using ABCA1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Hepg2 cells using ABCA1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Immunofluorescence analysis of Hela cells using ABCA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.