NEFH Primary Antibody
Item Information
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Size
Price
Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the heavy neurofilament protein. This protein is commonly used as a biomarker of neuronal damage and susceptibility to amyotrophic lateral sclerosis (ALS) has been associated with mutations in this gene.
Product Overview
Entrez GenelD
4744
Aliases
NFH; CMT2CC
Clone#
2C12F3
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human NEFH (AA: 2-251) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
ELISA
1/10000
References
1.PLoS One. 2019 Oct 1;14(10):e0222721. 2.J Neurol Neurosurg Psychiatry. 2018 Apr;89(4):367-373.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using NEFH mAb against human NEFH (AA: 2-251) recombinant protein. (Expected MW is 29.6 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using NEFH mAb against HEK293-6e (1) and NEFH (AA: 2-251)-hIgGFc transfected HEK293-6e (2) cell lysate.
IMMUNOHISTOCHEMISTRY
Figure 4: Immunohistochemical analysis of paraffin-embedded human cerebrum tissues using NEFH mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded human cerebellum tissues using NEFH mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using NEFH mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.