Mouse Monoclonal Antibody to NEDD9

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]

Product Overview

Entrez GenelD

4739

Aliases

CAS2; CASL; HEF1; CAS-L; CASS2

Clone#

3F7A9

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human NEDD9 (AA: 82-398) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Biomolecules.2018 Dec 10;8(4):169.2.Biochem Biophys Res Commun.2019 Jan 29;509(1):201-208.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using NEDD9 mAb against human NEDD9 (AA: 82-398) recombinant protein. (Expected MW is 37.3 kDa)

Western Blot

Figure 4:Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis