MUC16 Primary Antibody

Item Information
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Price
Description

This gene encodes a protein that is a member of the mucin family. Mucins are high molecular weight, O-glycosylated proteins that play an important role in forming a protective mucous barrier, and are found on the apical surfaces of the epithelia. The encoded protein is a membrane-tethered mucin that contains an extracellular domain at its amino terminus, a large tandem repeat domain, and a transmembrane domain with a short cytoplasmic domain. The amino terminus is highly glycosylated, while the repeat region contains 156 amino acid repeats unit that are rich in serines, threonines, and prolines. Interspersed within the repeats are Sea urchin sperm protein Enterokinase and Agrin (SEA) modules, leucine-rich repeats and ankyrin (ANK) repeats. These regions together form the ectodomain, and there is a potential cleavage site found near an SEA module close to the transmembrane domain. This protein is thought to play a role in forming a barrier, protecting epithelial cells from pathogens. Products of this gene have been used as a marker for different cancers, with higher expression levels associated with poorer outcomes.

Product Overview
Entrez GenelD
94025
Aliases
CA125
Clone#
4C8C8
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human MUC16 (AA: extra 1-200) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.BMC Cancer. 2019 Apr 30;19(1):406. 2.BMC Cancer. 2019 Feb 22;19(1):171.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using MUC16 mAb against human MUC16 (AA: 1-200) recombinant protein. (Expected MW is 23.8 kDa)
Figure 2: Western blot analysis using MUC16 mAb against human MUC16 (AA: 1-200) recombinant protein. (Expected MW is 23.8 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using MUC16 mAb against HEK293-6e (1) and MUC16 (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3: Western blot analysis using MUC16 mAb against HEK293-6e (1) and MUC16 (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.
FLOW CYTOMETRY
Figure 4: Flow cytometric analysis of SK-OV-3 cells using MUC16 mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of SK-OV-3 cells using MUC16 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MUC16 mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded liver cancer tissues using MUC16 mouse mAb with DAB staining.
IMMUNOHISTOCHEMISTRY
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MUC16 mouse mAb with DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using MUC16 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.