GATA3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.
Product Overview
Entrez GenelD
2625
Aliases
HDR; HDRS
Clone#
5H7C7
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human GATA3 (AA: (294-443)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200-1/1000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1,Int J Biol Sci. 2019 Sep 7;15(12):2522-2537;2,Arthritis Res Ther. 2019 Jun 25;21(1):156;3,Front Med. 2019 Dec;13(6):730-740.
Product Image
ELISA
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
Figure 2: Western blot analysis using GATA3 mAb against human GATA3 (AA: (294-443)) recombinant protein. (Expected MW is 36.7 kDa)
WESTERN BLOT
Figure 3: Western blot analysis using GATA3 mAb against HEK293-6e (1) and GATA3 (AA: (294-443))-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT
Figure 4: Western blot analysis using GATA3 mouse mAb against T47D (1) cell lysate.
FLOW CYTOMETRY
Figure 5: Flow cytometric analysis of Hela cells using GATA3 mouse mAb (green) and negative control (red).
IMMUNOHISTOCHEMISTRY
Figure 7: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using GATA3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.