4E-BP1 Primary Antibody
Item Information
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Price
Description
4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.
Product Overview
Entrez GenelD
1978
Aliases
BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316; EIF4EBP1
Clone#
4B6G10
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of 4E-BP1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Pause, A. et al. 1994.Nature. 371:762-767.
2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240-10247.
2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240-10247.
Product Image
Western Blot
Figure 1: Western blot analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein?1?and A431 cell lysate (2).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.