4E-BP1 Primary Antibody
Item Information
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Price
Description
4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1),also called ELF4EBP1/BP-1/PHAS-I ,which is located on chromosome 8p12, with 118-amino acid protein (about 13kDa). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non phosphorylated eIF4EBP1 will bind strongly to eIF4E while(24kDa), the phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.
Product Overview
Entrez GenelD
1978
Aliases
BP-1; 4EBP1; 4E-BP1; PHAS-I; MGC4316; EIF4EBP1
Clone#
9E12D9
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of 4EBP1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Pause, A. et al. 1994.Nature. 371:762-767.
2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240-10247.
2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240-10247.
Product Image
Western Blot
Figure 1: Western blot analysis using 4E-BP1 mouse mAb against truncated 4E-BP1 recombinant protein (1) and A431 cell lysate (2).
For Research Use Only. Not for use in diagnostic procedures.