MC38-EpCAM-LUC
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Description
MC38-EpCAM-LUC cells were generated from the C57BL/6 mouse colon cancer cell line MC38 by transduction with replication-defective lentivirus encoding human EpCAM and luciferase. Surface expression of EpCAM was confirmed by flow cytometry (Figure 1) and expression of luciferase was confirmed by luminescence after exposure to luciferin (Figure 2).
Product Overview
Composition
Suspension of cells in 0.5 ml - 1 ml of 90% FBS + 10% DMSO.
Storage
Store vial in liquid nitrogen immediately upon receipt.
Thawing
Partially immerse the vial in a 37°C water bath with gentle shaking until most of the medium is thawed. In a tissue culture hood, add 1 ml of pre-warmed culture medium into the vial and immediately transfer the contents of the vial to a centrifuge tube containing 5-10 ml of pre-warmed culture medium. Centrifuge the tube at room temperature for 5 minutes, aspirate the supernatant and suspend the cell pellet in 5-10 ml of pre-warmed culture medium.
Culture
MC38-EpCAM-LUC is an adherent cell line. Culture the cells in DMEM containing 10% FBS using a humidified incubator set to 5% CO2. When the cell monolayer is nearly confluent, use trypsin-EDTA to detach the cells from the culture flask and immediately neutralize the trypsin by adding fresh, pre-warmed culture medium to the cells. Cells should be passaged at least twice per week.
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Figure 1
PM-EPCAM-MC38-LUC cells were stained with an antibody specific for EPCAM and an isotype control antibody. The EPCAM antibody bound to the PM-EPCAM-MC38-LUC cells.
Figure 2
PM-EPCAM-MC38-LUC cells and PM-EPCAM-MC38 control cells were lysed and incubated with luciferin to assess luciferase expression. Only PM-EPCAM-MC38-LUC cells became luminescent.
For Research Use Only. Not for use in diagnostic procedures.