4T1-HER2
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Description
4T1-HER2 cells were generated from the BALB/c mouse breast cancer cell line 4T1 by transduction with replication-defective lentivirus encoding human HER2. Surface expression of HER2 was confirmed by flow cytometry (Figure 1).
Product Overview
Composition
Suspension of cells in 0.5 ml - 1 ml of 90% FBS + 10% DMSO.
Storage
Store vial in liquid nitrogen immediately upon receipt.
Thawing
Partially immerse the vial in a 37°C water bath with gentle shaking until most of the medium is thawed. In a tissue culture hood, add 1 ml of pre-warmed culture medium into the vial and immediately transfer the contents of the vial to a centrifuge tube containing 5-10 ml of pre-warmed culture medium. Centrifuge the tube at room temperature for 5 minutes, aspirate the supernatant and suspend the cell pellet in 5-10 ml of pre-warmed culture medium.
Culture
4T1-HER2 is an adherent cell line. Culture the cells in RPMI-1640 medium containing 10% FBS using a humidified incubator set to 5% CO2. When the cell monolayer is nearly confluent, use trypsin-EDTA to detach the cells from the culture flask and immediately neutralize the trypsin by adding fresh, pre-warmed culture medium to the cells. Cells should be passaged at least twice per week.
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Figure 1
PM-HER2-4T1 cells and parental 4T1 cells were stained with an antibody specific for HER2. The HER2 antibody bound to PM-HER2-4T1 cells but not to 4T1 cells.
For Research Use Only. Not for use in diagnostic procedures.