PC-3 ΔEpCAM
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Description
PC-3 ΔEpCAM cells were generated from the human prostate cancer cell line PC-3 by CRISPR followed by clonal selection. The loss of surface EpCAM expression was confirmed by flow cytometry (Figure 1).
Product Overview
Composition
Suspension of cells in 0.5 ml - 1 ml of 90% FBS + 10% DMSO.
Storage
Store vial in liquid nitrogen immediately upon receipt.
Thawing
Partially immerse the vial in a 37°C water bath with gentle shaking until most of the medium is thawed. In a tissue culture hood, add 1 ml of pre-warmed culture medium into the vial and immediately transfer the contents of the vial to a centrifuge tube containing 5-10 ml of pre-warmed culture medium. Centrifuge the tube at room temperature for 5 minutes, aspirate the supernatant and suspend the cell pellet in 5-10 ml of pre-warmed culture medium.
Culture
PC-3 ΔEpCAM is an adherent cell line. Culture the cells in RPMI-1640 containing 10% FBS using a humidified incubator set to 5% CO2. When the cell monolayer is nearly confluent, use trypsin-EDTA to detach the cells from the culture flask and immediately neutralize the trypsin by adding fresh, pre-warmed culture medium to the cells. Cells should be passaged at least twice per week.
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Figure 1
PM-KO-EPCAM-PC3 cells and parental PC-3 cells were stained with an antibody specific for EpCAM and an isotype control antibody. The EpCAM antibody bound to PC-3 cells but did not bind to PM-KO-EPCAM-PC3 cells.
For Research Use Only. Not for use in diagnostic procedures.