Jurkat-GFP
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Description
Jurkat-GFP cells were generated from the human T cell leukemia cell line Jurkat by transduction with replication-defective lentivirus encoding eGFP. Expression of eGFP was confirmed by flow cytometry (Figure 1).
Product Overview
Composition
Suspension of cells in 0.5 ml - 1 ml of 90% FBS + 10% DMSO.
Storage
Store vial in liquid nitrogen immediately upon receipt.
Thawing
Partially immerse the vial in a 37°C water bath with gentle shaking until most of the medium is thawed. In a tissue culture hood, add 1 ml of pre-warmed culture medium into the vial and immediately transfer the contents of the vial to a centrifuge tube containing 5-10 ml of pre-warmed culture medium. Centrifuge the tube at room temperature for 5 minutes, aspirate the supernatant and suspend the cell pellet in 5-10 ml of pre-warmed culture medium.
Culture
Jurkat-GFP is a cell line that grows in suspension. Culture the cells in RPMI-1640 medium containing 10% FBS using a humidified incubator set to 5% CO2. When the culture has reached a density of 2 million cells per ml, add a 10-fold volume of fresh, pre-warmed culture medium to the cells.
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Figure 1
PM-JURKAT-GFP cells and parental Jurkat cells were analyzed for GFP fluorescence by flow cytometry. Only the PM-JURKAT-GFP cells were fluorescent.
For Research Use Only. Not for use in diagnostic procedures.