A431 ΔROR1
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Description
A431 ΔROR1 cells were generated from the human skin cancer cell line A431 by CRISPR followed by clonal selection. The loss of surface ROR1 expression was confirmed by flow cytometry (Figure 1).
Product Overview
Composition
Suspension of cells in 0.5 ml - 1 ml of 90% FBS + 10% DMSO.
Storage
Store vial in liquid nitrogen immediately upon receipt.
Thawing
Partially immerse the vial in a 37°C water bath with gentle shaking until most of the medium is thawed. In a tissue culture hood, add 1 ml of pre-warmed culture medium into the vial and immediately transfer the contents of the vial to a centrifuge tube containing 5-10 ml of pre-warmed culture medium. Centrifuge the tube at room temperature for 5 minutes, aspirate the supernatant and suspend the cell pellet in 5-10 ml of pre-warmed culture medium.
Culture
A431 ΔROR1 is an adherent cell line. Culture the cells in DMEM containing 10% FBS using a humidified incubator set to 5% CO2. When the cell monolayer is nearly confluent, use trypsin-EDTA to detach the cells from the culture flask and immediately neutralize the trypsin by adding fresh, pre-warmed culture medium to the cells. Cells should be passaged at least twice per week.
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Figure 1
PM-KO-ROR1-A431 cells and parental A431 cells were stained with an antibody specific for ROR1 and an isotype control antibody. The ROR1 antibody bound to A431 cells but did not bind to PM-KO-ROR1-A431 cells.
For Research Use Only. Not for use in diagnostic procedures.