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PBMC-CLL (Chronic Lymphocytic Leukemia)
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IgG control measured with flow cytometry
Figure 3: Non-transduced cells have background IgG staining versus >60% CAR-T cells (Not shown).
|Description||PBMC (peripheral blood mononuclear cells) from CLL patient are isolated from blood samples using standard Ficoll-Paque and density gradient centrifugation. The PMBC cells can be used for isolation of T cells and other cell types. T cells can be used for generating CAR-T cells, for selection different subtypes of cells CD4+, CD8+ or others and used for different immunological experiments.|