CD19scFv-CD22scFv-TM-4-1BB-CD3z
Item Information
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Description
CD19scFv-CD22scFv-TM-4-1BB-CD3z CAR-T cells are generated with a lentivirus expressing CAR from normal human healthy donors. All cells are available at 1x10^6 to 1x10^7 cells in frozen vials, and shipped on dry-ice. For orders larger than >1x10^9, please inquire. CAR-T cells are cryopreserved in next-generation cryopreservation CS10 CryoStor medium (Sigma Aldrich, Catalog Number: C2874). All our cells are pathogen- and mycoplasma- free.
Product Overview
Cell Type Tested
T cells
Formulation
CS10 CryoStor Medium (Sigma Aldrich, Cat, #C2874)
Cell Viability
>90%
Mycoplasma Testing
The cells has been screened using a PCR-based mycoplasma detection kit to conirm the absence of mycoplasma species.
Storage
-80°C; liquid nitrogen
Shipping & Handing
This product will be shipped in a box containing dry ice at a temperature of -70°C.
Product Image
CAR Structure
Figure 1. Schematic representation of the scFv, costimulatory domain, and activation domain of PM-CAR1035. This construct targets CD19 + CD22.
FACS
Figure 2. FACS analysis with human FAB antibody detects CD22 CAR and FACS with CD19ScFv (FMC63, available from ProMab) shows expression of CD19-CAR.
RTCA
Figure 3. Top panel: RTCA cytotoxicity activity of effector CD19-CD22-4-1BB-CD3- CAR-T cells against Hela-CD19 target cells. This shows killing of CD19-positive target cells by CD19-CD22-CAR-T cells. Lower panel: RTCA cytotoxicity activity
For Research Use Only. Not for use in diagnostic procedures.