CASP9 Primary Antibody

Item Information
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Price
Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Product Overview
Entrez GenelD
842
Aliases
MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Clone#
5E8C8
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CASP9 (AA: 331-416) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Med Rep. 2018 Jul;18(1):1067-1073. 2.In Vivo. 2017 Mar-Apr;31(2):205-208.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CASP9 mAb against human CASP9 (AA: 331-416) recombinant protein. (Expected MW is 36 kDa)
Figure 2:Western blot analysis using CASP9 mAb against human CASP9 (AA: 331-416) recombinant protein. (Expected MW is 36 kDa)
Western Blot
Figure 3:Western blot analysis using CASP9 mAb against HEK293 (1) and CASP9 (AA: 331-416)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CASP9 mAb against HEK293 (1) and CASP9 (AA: 331-416)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometric
Figure 4:Flow cytometric analysis of Hela cells using CASP9 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Hela cells using CASP9 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.