ProMab Biotechnologies has years of experience providing CRO services with a skilled team of scientists who will work with you in developing your protein or peptide of interest. We offer protein expression services utilizing e. coli expression, baculovirus expression, mammalian expression, yeast expression, gene synthesis and peptide synthesis, as well.
Yeast expression can be advantageous especially for the large-scale production of eukaryotic recombinant proteins. Our two-phase system for recombinant protein Yeast expression takes 6-10 weeks from subcloning to expression in 2 phases for a total of $5,000
Phase I
Subcloning into Expression Vector
- Amplification/isolation of the gene of interest from a customer-supplied vector and subcloning into a suitable expression vector.
- Verification of the subcloned gene by restriction digest and sequencing.
- Preparation of the recombinant DNA construct.
- Transformation of recombinant constructs into a suitable Picha strain.
- Small scale induction to over-express target protein.
- Test for recombinant protein by SDS-PAGE and western blot (customer must provide appropriate antibodies)
Estimated time: 4-6 weeks
Price: $3,000
Phase II
Generation and Identification of Expression Positive Clones
- Large scale induction of clonal populations and 2.5L culture to over-express target protein.
- Ni-NTA purification of protein
- SDS-PAGE and WB confirmation of purified protein
- Delivery of purified protein in PBS or customer-specified buffer
Estimated time: 2 weeks
Price: $2,000
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