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Programmed death-ligand 1 (PD-L1) also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1) is a protein that in humans is encoded by the CD274 gene. Programmed death-ligand 1 (PD-L1) is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the adaptive arm of immune systems. PD-L1 binds to its receptor, PD-1, found on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition. PD-L1 binding to its receptor PD-1 on T cells delivers inhibitory signaling to TCR-mediated activation of IL-2 production and T cell proliferation. PD-L1 is shown to be highly expressed in a variety of malignancies, particularly lung cancer. In order to anticipate the effectiveness of gene therapy or systemic immunotherapy in blocking the PD-1 and PD-L1 checkpoints, PD-L1 might be employed as a prognostic marker and a target for anti-cancer immunity. The mRNA is generated by in vitro transcription (IVT) from linearized DNA template with CAR sequence. This IVT mRNA is capped using CleanCap, our proprietary co-transcriptional capping method, which results in the naturally occurring Cap 1 structure with high capping efficiency. It is polyadenylated and optimized for mammalian systems. It mimics a fully processed mature mRNA. The mRNA is embedded in proprietary LNP mix using microfluidics method and used for transfection of cells.

Product Overview
mRNA isolated in sodium citrate, sodium acetate, lipid mix in ethanol, and PBS.
Application & Handing
Store at 4°C. Work with mRNA-LNP on ice. It is important to minimize the time that the product spends at room temperature; after handling product during experiments, return immediately to ice. Upon receiving product, briefly pulse spin before opening to ensure product is at bottom of container; it is important not to spin for too long as this may rupture mRNA-LNP’s. Do not vortex. mRNA-LNP products should only be handled with certified RNAase-free reagents and consumables; use of filtered pipette tips is highly recommended.
4°C; ice
For Research Use Only. Not for use in diagnostic procedures.