CD49D Primary Antibody

Item Information
Catalog #
Size
Price
Description

The gene encodes a member of the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 4 subunit. This subunit associates with a beta 1 or beta 7 subunit to form an integrin that may play a role in cell motility and migration. This integrin is a therapeutic target for the treatment of multiple sclerosis, Crohn's disease and inflammatory bowel disease. Alternative splicing results in multiple transcript variants.

Product Overview
Entrez GenelD
3676
Aliases
ITGA4; IA4
Clone#
7E4C5
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD49D (AA: extra 833-977) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Leukemia. 2016 Oct;30(10):2011-2018.
2.Oncol Rep. 2015 Aug;34(2):779-86.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD49D mAb against human CD49D (AA: extra 833-977)) recombinant protein. (Expected MW is 42.6 kDa)
Figure 2:Western blot analysis using CD49D mAb against human CD49D (AA: extra 833-977)) recombinant protein. (Expected MW is 42.6 kDa)
Western Blot
Figure 3:Western blot analysis using CD49D mAb against HEK293 (1) and CD49D (AA: extra 833-977))-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD49D mAb against HEK293 (1) and CD49D (AA: extra 833-977))-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using CD49D mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of HL-60 cells using CD49D mouse mAb (green) and negative control (red).
Western Blot
Figure 5:Western blot analysis using CD49D mouse mAb against HL-60 (1) cell lysate.
Figure 5:Western blot analysis using CD49D mouse mAb against HL-60 (1) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.