Project & Orders
ProMab Biotechnologies' Cancer Stem Cell Media, PremiumTm has been developed as a specialized serum-free cancer stem cell media optimized for the growth and selection of spheroid forming cancer stem cells. The growth of tumor spheres from a variety of different cancer cell origins has been tested. Check out our additional line of CSC-products including cancer stem cell antibodies, and cancer stem cell markers.
Pluripotent, progenitor Cancer Stem Cell (CSC)’s, and their ability to direct the growth and proliferation of new tumor masses, even following therapeutic intervention, has led ProMab, with it’s long history of custom monoclonal antibody development to aggressively pursue programs to develop cancer stem cell marker-specific antibodies, that may then be used as research or direct therapeutic tools. A direct consequence of this strategy has led to ProMab’s development of cancer stem cell CSC-specific cell culture procedures, and products to allow for cancer stem cell (CSC) isolation and propagation as tumor spheres. This may be accomplished using ProMab Biotechnologies Cancer Stem PremiumTm media, a specialized serum-free media optimized for the growth and selection of spheroid forming cancer stem cells (CSC’s). As Cancer Stem Cell Media was developed specifically to examine both established and novel cell cancer stem cell (CSC)-specific surface markers, the user can be assured of its potency as a tool to allow for the analysis of the developing cancer stem cell (CSC) and associated tumorspheres.
Tumor Sphere Growth & Morphology
Morphology of tumor spheres, formed from adherent populations cultured in Cancer Stem Premium™ for 10 days.
Growth curves for cells disaggregated from tumorspheres up to 14 days, for a). the breast adenocarcinoma cell line MCF7, and b). the human hepatoma cell line PLC/PRF/5. In both cases tumorsphere re-formation is first observed at approximately 3-4 days, with maximum radius of spheres occurring at approximately 9-10 days. Cells were collected (graphs show mean values for triplicate well counts), trypsinized to disrupt aggregates and tumorspheres, and counted.
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