Baculovirus Expression System
Total Time: ~6-8 weeks
Promotion Price: US$4,500
Revised on Jan. 1, 2009

Call 1-866-339-0871
email: antibody@promab.com
FAQ   

Breakdown of Standard Expression:

Phase I * Amplification/isolation of the gene of interest out of a customer-supplied vector and subcloning of it into a transfer vector with His-tag. * Sequencing confirmation of insert. * Transformation of E. coli with baculoviral DNA and gene of interest. * Culture of E. coli and purification using mini-prep to produce bacmid (recombinant viral DNA). * Transfection of sf9 insect cells with bacmid. * Plaque purification and viral titer assay. * Small-scale culture to test expression level.  Estimated time: 4-6 weeks Price $2500 Phase II * Virus stock preparation * Large-scale culture and infection of insect cells for protein expression.  * Purification from 1 liter of infected insect cell using affinity column of Ni-NTA or other column. Estimated time: 2 weeks Price: $2000

Customers:

Abgent
Absalus, Inc.
Albert Einstein College of Medicine
Biochain Institute
Bio-Rad
Boston Univ. Goldman School of Dental Medicine
Cell Signaling Technology
College of Veterinary Medicine, Auburn University
Epitomics
Exelixis
Genemed Synthesis
Harvard Medical School
ImmunoVision
Invitrogen
Large Scale Biology Corp.
Montana State University
National Institute of Health
North Shore Long Island Jewish Health System
NYU
Panomics
Proteomtech
Qiagen
RDI
Santa Cruz Biotechnology
Texas Tech University Health Sciences Center
The Univ. of Texas Health Center at Tyler
Tularik
UC Berkeley
UC Davis
UCSF
University of  Michigan
University of Pennsylvania
University of Texas, Huston
Upstate
Yale University
Zyomyx