Protein Expression Service in CHO-K1 or HEK293  

Call  1-866-339-0871
email: Info@promab.com

Option 1: Transient Expression
Estimated time: 5~6 weeks
Price: $4,500
  • Guaranteed delivery of 3 mg or more protein if phase I is successful, otherwise only charge for phase I
    •
    Breakdown of Standard Expression by Phase:
    Phase I  

    * Cloning of your interest gene into vector with His-tag, or IgG-Fc tag.

    * Sequencing confirmation of insert.  

    * Small scale (5ml) transfection to test the expression level and optimization.

    Estimated time: 3-4 weeks

    Price $2,500 
    Phase II


    * Large scale transfection (500ml) of recombinant vector into HEK293 and protein expression, evaluation, and optimization.
        
    * Protein purification from 500ml of culture using affinity column of Ni-NTA or Protein G.
      
         The yield ranks from 3 to 25mg per 500ml with an average of 5mg.

    Estimated time: 2 weeks
    Price: $2,000

    Optional: Tag-cleavage.  $800

     
    Option 2:     Stable Cell line Expression- Milestone Service
    Estimated time: 10~14 weeks
    Price: $6,000~$8,000
  • Payment is due at the end of each phase
    •
     
    Breakdown of Standard Expression by Phase:
    Phase I   * Cloning of your interest gene into vector with His-tag, or IgG-Fc tag, or other selected tag

    * Sequencing confirmation of insert

    * Transfection of recombinant vector into CHO-k1

    * Generation and identification of cell expressing target protein

    Estimated time: 3 weeks
    Price: $1,500
     
    Phase II * Antibiotic selection

    * Cloning of expressing cell line, 2 cycles

    * Large-scale culture of stable cell line

    Estimated time: 5~8 weeks
    Price: $3,000 to $5,000
     
    Phase III * Purification from 1,000 ml of CHO-k1 culture using affinity column of Ni-NTA or Protein A.

    Estimated time: 2 to 3 weeks
    Price: $1,500

    Customers:

    Abcam
    Abgent
    Albert Einstein College of Medicine
    Alpha Diagnostic Intl. Inc.
    Amgen
    Amplified Proteomics
    Antagene, Inc.
    Assay Designs, Inc.
    Biocare Medical
    Biochain Institute
    Bio-Rad
    Boston Univ. Goldman School of Dental Medicine
    Cambridge Systems, Inc.
    Canadian Blood services
    Cell Signaling Technology
    CFIBR, Therapeutics
    Children's Hospital Boston
    Clontech Laboratories, Inc.
    College of Veterinary Medicine, Auburn University
    CPC Scientific, Inc.
    Evogenix Inc
    Exelixis
    Florida State University
    Genemed Synthesis
    Genentech, Inc.
    Georgetown University
    Harbor-UCLA Medical Center
    Harvard Medical School 
    ImmunoVision
    Invitrogen
    Jewish Gen Hospital
    M.D. Anderson Cancer Center
    Mayo Foundation
    Memorial University Health Medical Center
    Minerva Biotechnologies Corporation
    Montana State University 
    National Institute of Health
    Naval Medical Research Center
    New Jersey Medical School-UMDNJ
    New York University School of Medicine
    North Shore Long Island Jewish Health System
    Novus Biologicals, Inc
    NYU
    Panomics
    Pathway Diagnostics
    Proteomtech
    Qiagen
    R&D Systems Inc.
    RayBiotech, Inc.
    Remel Inc.
    SMART Biosciences, Inc.
    St. Michael's Hospital
    Stage I Diagnostics, Inc.
    Texas Tech University Health Sciences Center
    The Univ. of Texas Health Center at Tyler
    The Univ. Of Texas Health Center at Tyler
    U. North Carolina Hill
    U.of Louisville Health Sciences
    UC Berkeley
    UC Davis
    UC Santa Barbara 106
    UCSF 
    Univ. Texas-Health Sci Ctr at San Antonio
    University Health Network
    University of Central Florida
    University of Cincinnati
    University of Kentucky
    University of Liverpool
    University of Louisville School of Med
    University of Miami
    University of Missouri-columbia 
    University of Pennsylvania
    University of Texas, Huston
    University of Washington Medical Center
    University of  Michigan 
    Upstate
    UT Southwestern Medical Center at Dallas
    ViroStat, Inc.
    WRAIR/NMRC
    Wyeth Research
    Yale University School of Medicine

     


    ¡¡
    Copyright 2007 ProMab Biotechnologies, Inc. All Rights Reserved.
    5221 Central Avenue, Suite 200, Richmond, CA 94804, USA.  Tel: 1-866-339-0871.  Fax: 510-740-3625.  Email:     info@promab.com