ProMab Biotechnologies Inc.-Monoclonal Antibody Production service

Total Time: ~4 months.
Promotion Price: US$3,900
Revised on Jan. 1, 2009

Call 1-866-339-0871
email: antibody@promab.com
FAQ

ProMab Biotechnologies offers a reliable, speedy, and economical service for your custom monoclonal antibody needs.

Our monoclonal antibody development service gives you:

* Free peptide designing
* Immunization of 5 Balb/c mice
* Fusion of spleen cells with SP2/0 myeloma cells.
* ELISA screening for up to 3,000 clones.
* Free Western blot screening for protein antigen
* Guaranteed IgG isotype
* Progress Tracking
* ELISA positive guaranteed or your money back.

Breakdown of Standard Monoclonal Service by Phase:
Phase I	Immunization and boosts of 5 Balb/c mice with standard immunization protocol. ELISA evaluation of titer prior to selection for fusion. *Approximately 3-4 mg protein immunogen or 2 mg conjugated peptide immunogen and 0.5 mg free peptide are required.
Phase II	Once an acceptable titer is obtained, hybridoma fusion will be done using splenocytes from mouse with the best titer and myeloma cells. Supernatants from the growing hybridoma wells will be screened by ELISA. 10 clones will be sent to customer's lab for evaluation.
Phase III	*2 of these 10 clones selected by the customer will be subcloned by limiting dilution, isotyped,and expanded.

Additional Services:
Peptide synthesis and conjugation	US$500
Peptide synthesis without conjugation	US$350
Peptide conjugation	US$150
Additional clones (>10) in Phase II	US$50 per clone
Negative screening	US$300
Extra screening with IHC	US$300
Additional clones (>2) in Phase III	US$300 per clone
Cell-line adaptation to serum free medium	US$500~1,000
Cell banking and store in 10 ampules after first two months	US$30/month
Ascites production (<11 mice )	US$30/mouse+$200 setup fee
Ascites production (11-50 mice)	US$26/mouse+$200 setup fee
Ascites production (51-99 mice )	US$24/mouse+$200 setup fee
Ascites production (>99 mice )	US$20/mouse+$200 setup fee
antibody purification by Protein G or ammonium persulfate for IgM (10 mice minimum)	US$30/mouse
Antibody culture in bioreactor, per liter	US$1,100
Antibody culture in flask, per liter	US$800
Antibody purification by protein G from one liter of medium	US$300
Antibody purification by protein G from ascites (20mg)	US$300
Antibody epitope mapping by peptide library	US$1,200
Antibody conjugation with FITC, per mg	US$600
Antibody conjugation with HRP, per mg	US$600
Antibody biotinylation, per mg	US$600

Our client retains all property rights to selected hybridomas and monoclonal antibodies developed in their project. Each project is conducted with complete confidentiality.

Customers:

Abcam

Abgent

Albert Einstein College of Medicine

Alpha Diagnostic Intl. Inc.

Amgen

AMPLIFIED PROTEOMICS

Antagene, Inc.

Assay Designs, Inc.

Biocare Medical

Biochain Institute

Bio-Rad

Boston Univ. Goldman School of Dental Medicine

Cambridge Systems, Inc.

Canadian Blood services

Cell Signaling Technology

CFIBR, Therapeutics

Children's Hospital Boston

Clontech Laboratories, Inc.

College of Veterinary Medicine, Auburn University

CPC Scientific, Inc.

Evogenix Inc

Exelixis

Florida State University

Genemed Synthesis

Genentech, Inc.

Georgetown University

Harbor-UCLA Medical Center

Harvard Medical School

ImmunoVision

Invitrogen

Jewish Gen Hospital

M.D. Anderson Cancer Center

Mayo Foundation

Memorial University Health Medical Center

Minerva Biotechnologies Corporation

Montana State University

National Institute of Health

Naval Medical Research Center

New Jersey Medical School-UMDNJ

New York University School of Medicine

North Shore Long Island Jewish Health System

Novus Biologicals, Inc

NYU

Panomics

Pathway Diagnostics

Proteomtech

Qiagen

R&D Systems Inc.

RayBiotech, Inc.

Remel Inc.

SMART Biosciences, Inc.

St. Michael's Hospital

Stage I Diagnostics, Inc.

Texas Tech University Health Sciences Center

The Univ. of Texas Health Center at Tyler

The Univ. Of Texas Health Center at Tyler

U. North Carolina Hill

U.of Louisville Health Sciences

UC Berkeley

UC Davis

UC Santa Barbara 106

UCSF

Univ. Texas-Health Sci Ctr at San Antonio

University Health Network

University of Central Florida

University of Cincinnati

University of Kentucky

University of Liverpool

University of Louisville School of Med

University of Miami

University of Missouri-columbia

University of Pennsylvania

University of Texas, Huston

University of Washington Medical Center

University of Michigan

Upstate

UT Southwestern Medical Center at Dallas

ViroStat, Inc.

WRAIR/NMRC

Wyeth Research

Yale University School of Medicine