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Your project will be managed by a team with extensive experience in the expression and purification of a wide range of proteins in both bacterial and baculovirus expression systems. All of our services are fully supported by scientists who directly manage your project and are on hand to develop and support a service that meets your exact requirements.

Breakdown of E.coli Expression System by Phase:

Total Time: 4-6 weeks Price: US $ 1,800 *3mg protein guaranteed
Phase I	Amplification/isolation of the gene of interest out of a customer-supplied cDNA and subcloning it into a bacterial expression vector Verification of the authenticity of the subcloned gene by restriction enzyme digest and sequencing. Estimated time: 2-3 weeks Price: $800
Phase II	Transformation of recombinant constructs into high-efficiency expression bacterial strain Mini-induction to over-express the target protein *Test for the expression of the recombinant protein by western blot (customer must provide appropriate antibodies) if desired Estimated time: 1 weeks Price: $400
Phase III	*Protein purification using Ni-NTA (for 6xHIS-tagged proteins) or glutathione (for GST-tagged proteins) beads at either native or denatured conditions Estimated time: 1 to 2 weeks Price: $600

Breakdown of Baculovirus Expression System by Phase:

Total Time: 8-10 weeks Price: US $ 4,500 *Payment is due only for the phase that has been successfully completed
Phase I	* Cloning of your interest gene into a baculoviral transfer vector with His-tag, IgG Fc-tag, or other selected tag * Sequencing confirmation of insert * Transfection of recombinant vector and baculovirus DNA * Generation and identification of baculoviral strain expressing target protein Estimated time: 3 weeks Price: $1,500
Phase II	* Plaque purification of baculoviral strain expressing target protein * Amplification of recombinant baculovirus for high titer stock * Large-scale culture and infection of insect cell for protein expression Estimated time: 4~5 weeks Price: $2,000
Phase III	* Purification from 1,000 ml of infected insect cell using affinity column of Ni-NTA or Protein A Estimated time: 1 to 2 weeks Price: $1,000
Clients: Abgent Albert Einstein College of Medicine Biochain Institute Bio-Rad Boston Univ. Goldman School of Dental Medicine Cell Signaling Technology College of Veterinary Medicine, Auburn University Epitomics Exelixis Genemed Synthesis Harvard Medical School ImmunoVision Invitrogen Large Scale Biology Corp. Montana State University National Institute of Health North Shore Long Island Jewish Health System NYU Panomics Proteomtech Qiagen RDI Santa Cruz Biotechnology Texas Tech University Health Sciences Center The Univ. of Texas Health Center at Tyler Tularik UC Berkeley UC Davis UCSF University of Michigan University of Pennsylvania University of Texas, Huston Upstate Yale University Zyomyx ...