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Phase I Cloning of your interest gene into bacterial expression vector with His-tag or other selected tag (such as GST or thioredoxin).

*Amplification of gene of interest out of a customer-supplied vector and subcloning of it into an expression vector.

*Verification of the authenticity of the subcloned gene by restriction enzyme digestion and sequencing.

*Transformation of recombinant construct into high efficiency expression bacterial strain.

*Mini-induction to over-express the target protein.

*Test for the expression of the recombinant protein by SDS-PAGE or/and western blot.

Estimated time: 2~4 weeks
Price: $1,200

Phase II Large-scale culture and purification

*One liter of bacterial culture will be induced with IPTG and harvested for protein purification.

*Protein purification using Ni-NAT (for His-tagged proteins) or glutathione (for GST-tagged proteins) beads at either native or denatured conditions.

* Solubility not guaranteed

Estimated time: 2 weeks
Price: $600

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Customers:

Abgent
Absalus, Inc.
Albert Einstein College of Medicine
Biochain Institute
Bio-Rad
Boston Univ. Goldman School of Dental Medicine
Cell Signaling Technology
College of Veterinary Medicine, Auburn University
Epitomics
Exelixis
Genemed Synthesis
Harvard Medical School
ImmunoVision
Invitrogen
Large Scale Biology Corp.
Montana State University
National Institute of Health
North Shore Long Island Jewish Health System
NYU
Panomics
Proteomtech
Qiagen
RDI
Santa Cruz Biotechnology
Texas Tech University Health Sciences Center
The Univ. of Texas Health Center at Tyler
Tularik
UC Berkeley
UC Davis
UCSF
University of  Michigan
University of Pennsylvania
University of Texas, Huston
Upstate
Yale University
Zyomyx