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CD106

Order Information
Catalog# Size/Concentration Price
31490 1.0mg/ml $341.00
Specification
Entrez GeneID 7412
Aliases VCAM1; INCAM-100
Clone# 6A11D8
MW 81.3kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human; Monkey; Rat;
Immunogen Purified recombinant fragment of human CD106 (AA: extra 25-183) expressed in E. Coli.
Formulation Purified antibody in PBS with 0.05% sodium azide
Application
WB 1/500 - 1/2000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
Description
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
References
1.Stem Cell Res Ther. 2016 Apr 4;7:49.
2.Cell Signal. 2015 Dec;27(12):2467-73.
Sequence
extra(25-183)
Related Products

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CD106 mAb against human CD106 (AA: extra 25-183) recombinant protein. (Expected MW is 43.6 kDa)

Western Blot

Figure 3:Western blot analysis using CD106 mAb against HEK293 (1) and CD106 (AA: extra 25-183)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using CD106 mouse mAb against EC (1), COS7 (2), MCF-7 (3), HepG2 (4), and Hela (5) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using CD106 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 6:Flow cytometric analysis of HL-60 cells using CD106 mouse mAb (green) and negative control (red).