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Order Information
Catalog# Size/Concentration Price
31374 1.0mg/ml $341.00
Entrez GeneID 84868
Aliases HAVCR2; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
Clone# 7C8A7
MW 33.4kDa
Host/Isotype Mouse IgG1
Storage 4℃; -20℃ for long term storage
Species Reactivity Human;
Immunogen Purified recombinant fragment of human TIM3 (AA: extra 22-202) expressed in HEK293 cells.
Formulation Purified antibody in PBS with 0.05% sodium azide
WB 1/500 - 1/2000
ICC 1/200 - 1/1000
FCM 1/200 - 1/400
ELISA 1/10000
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
1.Tumour Biol. 2016 Jun;37(6):8209-18.
2.Int Immunopharmacol. 2015 Dec;29(2):635-641.
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Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using TIM3 mAb against human TIM3 (AA: extra 22-202) recombinant protein. (Expected MW is 50 kDa)

Immunofluorescence analysis

Figure 3:Immunofluorescence analysis of Hela cells using TIM3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Figure 4:Flow cytometric analysis of Jurkat cells using TIM3 mouse mAb (green) and negative control (red).